Specimen:

Fetal Sample (Amniotic or Chorionic Villus), Parental Samples (Whole Blood)

Collection Medium:

Minimum:

Fetal Sample:
 Amniotic Fluid (AF)    1 ml per week gestational age
 Chorionic Villus (CV)  10 mg clean villus
Parental Sample(s)      3 ml whole blood in EDTA tube

Analytic Time:

Turnaround time for results is 4 to 7 weeks.

Reference Range:

Normal

Comments:

Fetal Sample:  Amniotic Fluid and Chorionic Villi samples must remain 
at room temperature at all times and be shipped immediately after 
collection.  Place Amniotic Fluid in a sterile centrifuge tube.  Place 
Chorionic Villi Samples in transport tube containing enough tissue 
culture medium to cover the entire sample.   Do not allow tissue to 
become dry.

Fetal samples must be shipped for overnight delivery, Monday through 
Wednesday only.  Parental sample(s) may be kept at room temperature for 
24 hours.  Refrigerate if overnight, weekends and holidays.

Contact the Cytogenetics laboratory at 319-356-3877 and provide name, 
institution name, telephone number, patient name and the tracking 
number for the package.  Sites will be notified upon receipt of samples 
if they are found to be suboptimal.  In addition, site will be notified 
within one week of receipt if the specimens failed to provide optimal 
growth.

Test
Limitations:

Contact the Cytogenetics Laboratory at 319-356-3877 and provide 
information on when fetal sample is going to be collected.

Methodology:

Southern Blot

Sample
Processing:

Lavender tube is sent as whole blood, no processing necessary.

Sample
Storage:

Room temperature for up to 24 hours, then refrigerate the whole blood 
if it is necessary to be held overnight, or weekends, or holidays.

Transport
Instructions:

Place specimen into styrofoam container, seal container.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.
Recommend express mail or equivalent if not on courier service.

CPT Code:

83890(x3), 83892(x6), 83894(x3), 83896(x3)
83897(x3), 83912(x3), 88235, 88240

See Additional Information:
Facioscapulohumeral Dystrophy (FSHD) Sample Requirements for Prenatal Testing