Specimen:

Plasma

Collection Medium:

Minimum:

2 mL plasma.  HCV molecular testing cannot be added onto a previously 
opened vacutainer tube.  Testing requires a dedicated collection tube.

Rejection Criteria:

+++++++++++++

Testing
Schedule:

Once per week

Analytic Time:

1 hour (upon receipt in laboratory)
++++++++++++++++++

Reference Range:

Analytic Measurement Range:  200-10,000,000 IU/mL
Results Reported:  Negative, <200 IU/ml, 200 and greater IU/mL

Interpretive Data:

++++++++++++++++++++

Comments:

Record draw time.

All collection tubes need to be processed within six hours of 
collection.  If specimens cannot reach the Microbiology laboratory at 
UIHC within the six hours, process as follows:

White top tube:
Centrifuge for 10 minutes at 800-1600 x g.  Do not pour off plasma, 
maintain sterility and transport to the laboratory on ice within 24 
hours.

Test
Limitations:

+++++++++++++++++++

Methodology:

Homogeneous Polymerase Chain Reaction (real time PCR)

Sample
Processing:

Within 6 hours of collection, centrifuge at 800-1600 x g for 10 
minutes.
Label transport tube with two patient identifiers, date and time of 
collection.
Maintain sterility and forward promptly to lab.
Specimen should be collected and packaged as close to shipping time as 
possible.

Sample
Storage:

Refrigerate.
See "Instructions:" below for special storage requirements.

Transport
Instructions:

Place coolant packs into styrofoam container.
Place requisition into outside pocket of bag.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.
Recommend express mail or equivalent if not on courier service.

Instructions:

Cannot use specimen for other testing.  Must be received within 24 
hours of collections.

CPT Code:

87522