University of Iowa
Diagnostic Laboratories
(UIDL) Test Directory

319-384-7212 (local)
1-866-844-2522 (toll free)


Hepatitis C Genotype Order Code: HCVGENO
Order Form: Molecular Oncology & Infectious Disease Requisition
Specimen:
Plasma
Collection Medium:
White top plasma preparation tube (PPT, EDTA)
Minimum:
Minimum 2.0 mL plasma required.  Both the genotyping and the 
quantitative assay can be performed from this 2.0 mL.  Sample tube must 
remain sterile.  HCV molecular testing cannot be added onto a 
previously opened vacutainer tube.  Testing requires a dedicated 
collection tube.
Testing
Schedule:
Once per week
Analytic Time:
1 week
Comments:
Test is performed once per week.
Methodology:
Homogeneous Polymerase Chain Reaction (real time PCR)
Sample
Processing:
Within 6 hours of collection, centrifuge at 800-1600 x g for 10 
minutes.
Label transport tube with two patient identifiers, date and time of 
collection.
Maintain sterility and forward promptly to lab.
Specimen should be collected and packaged as close to shipping time as 
possible.
Sample
Storage:
Refrigerate.
See "Instructions:" below for special storage requirements.
Transport
Instructions:
Place coolant packs into styrofoam container.
Place requisition into outside pocket of bag.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.
Recommend express mail or equivalent if not on courier service.
Instructions:
Must be received within 24 hours of collection.
CPT Code:
87902

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Updated: 04/16/2007