The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Factor 5 Leiden/Factor 2 Gene PCR Assay
Order Code: PROTPCR
Epic Lab Code: LAB346
Order Form: A-1a Miscellaneous Request or Epic Req
  Molecular Pathology
6004 BT GH
384-9568
Specimen:
Whole Blood
Collection Medium:
Lavender top tube (EDTA)
Minimum:
Adult mimimum: 3 mL whole blood in lavender top tube (EDTA)
Children minimum: 2 mL whole blood in lavender top tube (EDTA)
Testing on smaller volumes than those requested will be attempted.  
However, in some cases, small blood volumes may compromise the ability 
to perform testing.
Testing requires a dedicated collection tube.
Delivery Instructions:
Room temperature up to 24 hours.  Refrigerate overnight, 
weekends and holidays.
Testing Schedule:
Weekly
Analytic Time:
14 days
Reference Range:
Normal
Comments:
Genomic DNA is PCR amplified and the presence of the Factor 5 Leiden 
and Factor 2 mutations associated with venous thrombosis are assessed 
simultaneously by gel electrophoresis.
Methodology:
Polymerase Chain Reaction (PCR) - Amplification Refractory Mutation 
Systems (ARMS)
CPT Code:
83890, 83900(x2), 83894, 83912

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Updated: 09/29/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.