The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Direct Antiglobulin Test
Order Code: DC
Epic Lab Code: LAB4360
Order Form: DeGowin Blood Center Requisition
  Blood Bank - DeGowin Blood Center
C271 GH
356-2561
Specimen:
Blood
Collection Medium:
or
Pink top tube (EDTA sprayed) Lavender top tube (EDTA)
Minimum:
Adults - 2 mls
Pediatrics - 1 ml or EDTA microtainer
Rejection Criteria:
Specimen must be labeled with patient's first and last name and medical 
record number.  Specimens will be rejected if information is not on the 
label when received.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
2 hours (upon receipt in laboratory)
Reference Range:
Negative result means that no antibodies were detected on the patient's
red cells using polyspecific antiglobulin technique.
Comments:
Monospecific testing for IgG and C3 complement is automatically
performed when the polyspecific test is positive.

Elution performed per pathologist recommendation or clinician order.

Only monospecific testing of IgG will be performed on cord samples when 
mothers are alloimmunized or when mothers antibody status is unknown.
Methodology:
Tube test
CPT Code:
86880

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Updated: 09/22/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.