Specimen

Urine More information

Collection Medium:

Minimum:

24 hour collection to yield 5 mL aliquot from well mixed 24 hr sample.
Pediatric minimum is 1 mL aliquot from well mixed 24 hr sample.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after
collection period is completed.

Specimen
Instructions:

Record start and end of 24 hour collection on requisition. Urine must 
be refrigerated during collection.

Analytic Time:

2 working days (upon receipt)

Reference Range:

Test Name               Age Group      Gender       Reference Interval
Cortisol (ug/day)    3 -  8 years   Female & Male        <18 ug/day
                     9 - 12 years   Female & Male        <37 ug/day
                    13 - 17 years   Female & Male        <56 ug/day
               18 years and older   Female only          <45 ug/day
               18 years and older   Male only            <60 ug/day

Cortisol/Cortisone Ratio*
                     0 - 11 years   Female & Male      To be determined
               12 years and older   Female & Male      0.15 - 0.50

Cortisone      Value used to calculate Cortisol/Cortisone ratio

Cortisol (ug/g creatinine)
                     0 -  2 years   Female & Male        <120 ug/g
                     3 -  8 years   Female & Male        < 90 ug/g
                     9 - 12 years   Female & Male        < 55 ug/g
                    13 - 17 years   Female & Male        < 42 ug/g
               18 years and older   Female & Male        < 85 ug/g

Reference intervals are based on literature from Tayor RL et al.  
Validation of a High-Throughput Liquid Chromatography-Tandem Mass 
Spectrometry Method for Urine Cortisol and Cortisone, Clinical 
Chemistry 2002: 48(9):1511-1519.

Comments:

* The ratio of the concentrations of cortisol to cortisone should not 
be evaluated if the cortisol concentration is < 5 ug/L.
The urine cortisol to creatinine ratio will be reported whenever the 
urine collection is random, or less than 20 hours, or the urine volume 
is less than 400 mL/24 hours.

Methodology:

Tandem Mass Spectrometry (LC-MS/MS)

CPT Code:

Cortisol 82530
LC-MS/MS 83789

See Additional Information:
Collection and Preservation of 24-Hour Urine Specimens
Urine Tests Requiring Preservatives, Refrigeration or Special Containers