The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


CBC with Automated Differential
Order Code: CBCD
Epic Lab Code: LAB293
Order Form: A-1a General Lab or Epic Req
  Hematology
6240 RCP
356-3527
Specimen:
Whole Blood
Collection Medium:
Lavender top tube (EDTA)
Minimum:
Full draw; any size lavender top or fingerstick.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
Routine turn around time is approximately 2.5 hours.
Reference Range:
                         Adults         Children (5-18 yrs)
                          (mm3)                (mm3)
Band Neutrophils          -                   -
Neutrophils           2188-7800/mm3       1700-7500/mm3
Eosinophils             40-390/mm3          40-650/mm3
Basophils               10-136/mm3           7-140/mm3
Monocytes              130-860/mm3          28-825/mm3
Lymphocytes            875-3300/mm3       1250-3380/mm3
Immature Granulocytes     -                   -
Comments:
A CBC must be ordered to perform a differential.  Hematology 
instruments are automated differential screening instruments that 
identify WBC's by their size and staining activity.  It performs a 
complete cell identification that includes neutrophils, lymphocytes, 
monocytes, basophils and eosinophils. The automated instruments count 
32,000 white blood cells and statistically provide a more accurate 
differential count than a manual differential.  All differentials will 
be analyzed on the instrument first.  If the results fail the screening 
criteria, a manual differential will be performed.  Pathologist 
approval is required to perform a manual differential on a specimen 
with an automated differential that passed the screening criteria.
Methodology:
Flow Cytometry
Auto Diff Cytochemical Staining
Manual Diff Wright's Stain
CPT Code:
85025
 
See Additional Information:
Hematology Pediatric Reference Ranges

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Updated: 11/25/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.