Specimen:

Whole Blood

Collection Medium:

Minimum:

Adult Minimum:  20 mL whole blood and 20 mL normal control in sterile 
containers at 20-25 Degrees C.

Pediatric Minimum:  3 mL whole blood (green, sodium heparin) and 3 mL 
whole blood (green, sodium heparin) normal control from a healthy 
unrelated individual in sterile containers at 20-25 Degrees C.

Rejection Criteria:

Refrigerated or frozen specimens and specimens in transport longer than 
48 hours. Specimens submitted in yellow (ACD Solution B).

Delivery Instructions:

Submit specimen to laboratory as soon as possible after collection.

Specimen
Instructions:

Two 10 mL yellow (ACD Solution A) and two normal controls in 10 mL 
yellow (ACD Solution A) tubes from a healthy unrelated individual.

LIVE CELLS REQUIRED. CRITICAL AMBIENT.

Analytic Time:

within 10 days

Reference Range:

By report

Interpretive Data:

Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, 
and tetanus antigen are tested independently in mononuclear cell 
culture. Cell proliferation in response to mitogens and antigens is 
determined by 3H-thymidine incorporation. Cytokine production in 
response to mitogens is determined by multi-analyte fluorescence 
detection.

Proliferation results are reported as the mitogen and/or antigen 
stimulated counts per minute (CPM) and a stimulation index (SI) which 
represents the ratio of the stimulated lymphocyte CPM to an 
unstimulated control CPM. Cytokine results are reported in pg/mL.

Results are to be used for research purposes or in attempts to 
understand the pathophysiology of immune, infectious, or inflammatory 
disorders.

Comments:

Interpretation comparing the patient results to the simultaneously 
collected client normal control and the laboratory normal control will 
be provided by the reference laboratory medical director.

Methodology:

Cell Culture/Multi-Analyte Fluorescence Detection

CPT Code:

86353 x 5 Lymphocyte transformation; 83520 x 12 Cytokines