Specimen:

Plasma or CSF

Collection Medium:

Alternate
Collection Media:

Pink top tube (EDTA sprayed), CSF container

Minimum:

Preferred minimum:  4 mL lavender (EDTA) or pink (K2EDTA), or CSF
Absolute minimum:  1 mL plasma or CSF in a sterile container
Pediatric minimum:  0.25 mL plasma or CSF in a sterile container (Less
                    volume may decrease sensitivity of assay.)

Rejection Criteria:

Nonfrozen samples, samples exposed to repeated freeze/thaw cycles, 
nonsterile or leaking containers, heparinized samples, and hemolyzed 
samples.

Delivery Instructions:

Deliver to laboratory within 1 hour of collection.

Analytic Time:

1 week

Reference Range:

Negative - Enterovirus nucleic acid not detected by RT-PCR
Positive - Enterovirus nucleic acid detected by RT-PCR

Interpretive Data:

A negative result does not rule out the presence of PCR inhibitors in 
the patient specimen or Enterovirus nucleic acid in concentrations 
below the level of detection of the assay.

Methodology:

Reverse Transcription/Polymerase Chain Reaction

CPT Code:

87498