Collection Medium:

Minimum:

Adults:  One 3 mL green (sodium heparin) and one normal control in a 3 
mL green (sodium heparin) from a healthy unrelated individual.

Pediatrics:  3 mL whole blood (green, sodium heparin) and 3 mL whole 
blood (green, sodium heparin) normal control from a healthy unrelated 
individual.

Rejection Criteria:

Refrigerated or frozen samples or samples in transport longer than 48 
hours.

Delivery Instructions:

CRITICAL AMBIENT. Do not refrigerate or freeze.

Specimen
Instructions:

Patient and control samples must be collected within 48 hours of test 
performance.

Analytic Time:

5 days upon receipt at reference laboratory

Reference Range:

By report

Interpretive Data:

White blood cells are incubated with dihydrorhodamine 123 (DHR) and 
catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA).  
Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the 
cell is measured by flow cytometry.

Results are reported as the ratio of the mean channel fluorescence of 
stimulated cells versus unstimulated cells, which yields a stimulation 
index (SI).

Comments:

If sample shows abnormal results when stimulated, and no control was 
sent, test should be resubmitted with control sample to validate the 
conditions of collection, processing and transport.

Methodology:

Flow Cytometry

CPT Code:

88184 First marker; 88185 Additional marker; 88187 Interpretation 2-8 
markers