The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Dengue Fever Virus Antibodies, IgG & IgM
Order Code: DENGUE
Epic Lab Code: LAB2988
Order Form: A-1a Miscellaneous Request or Epic Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen:
Serum
Collection Medium:
Red top tube
Minimum:
Adult Preferred Minimum:  1 mL serum
Adult Absolute Minimum:  0.3 mL serum
Pediatric Minimum:  0.1 mL serum
Rejection Criteria:
Severely lipemic, contaminated, heat-inactivated, or hemolyzed 
specimens.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after
collection period is completed.
Analytic Time:
2 weeks
Reference Range:
Dengue Fever Virus Antibody, IgG

1.64 IV or less: Negative - No significant level of detectable dengue
                            fever virus IgG antibody.

1.65-2.84 IV: Equivocal - Questionable presence of antibodies.  Repeat
                          testing in 10-14 days may be helpful.

2.85 IV or greater: Positive - IgG antibody to dengue fever virus
                               detected, which may indicate a current
                               or past infection.

Dengue Fever Virus Antibody, IgM

1.64 IV or less: Negative - No significant level of detectable dengue
                            fever virus IgM antibody.

1.65-2.84 IV: Equivocal - Questionable presence of antibodies.  Repeat
                          testing in 10-14 days may be helpful.

2.85 IV or greater: Positive - IgM antibody to dengue fever virus
                               detected, which may indicate a current
                               or recent infection.

However, low levels of IgM antibodies may occasionally persist for more 
than 12 months post-infection.
Comments:
Acute anc convalescent specimens must be labeled as such paralled 
testing is preferred and convalescent sample must be received within 30 
days of acute.
Methodology:
Enzyme-Linked Immunosorbent Assay
CPT Code:
86790(x2)

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Updated: 02/25/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.