Specimen:

Serum

Collection Medium:

Minimum:

Preferred Adult Minimum:  1.0 mL serum
Absolute Adult Minimum:  0.2 mL serum

Rejection Criteria:

Severely lipemic, contaminated, heat-inactivated, or hemolyzed 
specimens.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after
collection period is completed.

Analytic Time:

2 weeks

Reference Range:

1.64 IV or less: Negative - No significant level of detectable dengue
                            fever virus IgG antibody.

1.65-2.84 IV: Equivocal - Questionable presence of antibodies.  Repeat
                          testing in 10-14 days may be helpful.

2.85 IV or greater: Positive - IgG antibody to dengue fever virus
                               detected, which may indicate a current
                               or past infection.

Interpretive Data:

Patients in the early stage of dengue fever virus infection may not 
have detectable IgG antibodies, as the IgG response may take several 
weeks to develop.  In the absence of detectable IgG, testing for IgM 
class antibody is strongly recommended.  The best evidence for current 
infection is a significant change on two appropriately timed specimens, 
where both tests are done in the same laboratory at the same time.

Comments:

Acute and convalescent specimens must be labeled as such paralled 
testing is preferred and convalescent sample must be received within 30 
days of acute.

Methodology:

Enzyme-Linked Immunosorbent Assay

CPT Code:

86790