Specimen:

Serum

Collection Medium:

Minimum:

Adult Preferred Minimum:  1.0 mL serum
Absolute Adult Minimum:  0.2 mL serum

Rejection Criteria:

Severely lipemic, contaminated, heat-inactivated, or hemolyzed 
specimens.

Delivery Instructions:

Submit specimen to laboratory as soon as possible after
collection period is completed.

Analytic Time:

2 weeks

Reference Range:

1.64 IV or less: Negative - No significant level of detectable dengue
                            fever virus IgM antibody.

1.65-2.84 IV: Equivocal - Questionable presence of antibodies.  Repeat
                          testing in 10-14 days may be helpful.

2.85 IV or greater: Positive - IgM antibody to dengue fever virus
                               detected, which may indicate a current
                               or recent infection.

However, low levels of IgM antibodies may occasionally persist for more 
than 12 months post-infection.

Interpretive Data:

The best evidence for current infection is a significant change on two 
appropriately timed specimens, where both tests are done in the same 
laboratory at the same time.

Comments:

Acute and convalescent specimens must be labeled as such; parallel 
testing preferred.  Convalescent sample must be received within 30 ays 
of acute.

Methodology:

Enzyme-Linked Immunosorbent Assay

CPT Code:

86790