The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK

HLA Genotyping DR Beta 1, DR Beta 3, 4, 5 or DQ Beta and DP Intermediate Resolution (VAMC)

Order Form: Tissue Typing Laboratory Test Requisition 		  Iowa Regional Histocompatibility and Immunogenetics
Veterans Affairs Hospital 10E-19
(319-338-0581), EXT. 5640
dial 158 from UIHC 
Specimen:
 
Whole Blood
Minimum:
 
THREE - FOUR 10 mL yellow top (ACD) tubes.  For patients with low white 
counts-additional tubes are needed.  Buccal swabs may be used if normal 
sample requirements can not be met.
Delivery Instructions:
 
Deliver at room temperature.
Analytic Time:
 
10 days
Comments:
 
Order each allele separately.
Methodology:
 
Polymerase Chain Reaction (PCR) - Sequence Specific Oligonucleotide 
(SSO)
CPT Code:
 
86891(x1) each
83894(x1) each
83900(x1) each
83912(x2) each
83896(x72) DR Beta 1        Use modifier (4E)
83896(x39) DR Beta 3, 4, 5  Use modifier (4E)
83896(x47) DQ Beta          Use modifier (4F)
83896(x39) DP Beta          Use modifier (4G)
 
See Additional Information:
Iowa Regional Histocompatibility and Immunogenetics Laboratory Required Content on Requisitions
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Updated: 04/15/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.