The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


5-Flucytosine, Antimicrobial Assay
Order Code: 5FLUC
Epic Lab Code: LAB2512
Order Form: A-1a Miscellaneous Request or Epic Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen:
Serum, Plasma, Spinal Fluid
Collection Medium:
Lavender top tube (EDTA)
Minimum:
Submit only 1 of the following specimens:

Plasma - Draw blood in a lavender-top (EDTA) tube(s).  Send 1 mL Plasma 
for a peak level should be drawn 30 minutes after completion of 
infusion of an intravenous dose or 60 minutes after an intramuscular or 
oral dose of the antimicrobial to be assayed.  (Absolute Plasma 
Minimum:  0.3 mL)

Serum - Draw blood in a plain, red-top tube(s).  Send 1 mL Serum for a 
peak level should be drawn 30 minutes after completion of infusion of 
an intravenous dose or 60 minutes after an intramuscular or oral dose 
of the antimicrobial to be assayed.  (Absolute Serum Minimum:  0.3 mL)

Spinal Fluid - 1.0 mL of spinal fluid. Send specimen frozen in plastic 
vial.  (Absolute CSF Minimum:  0.3 mL)
Rejection Criteria:
Gel separator tubes.
Analytic Time:
5 days upon receipt at reference laboratory
Reference Range:
PLASMA OR SERUM
  Peak:  100 ug/mL
  Trough:  50 ug/mL
SPINAL FLUID
  No established reference values
Comments:
Indicate specimen type on request form and label specimen 
appropriately.
Methodology:
High-Performance Liquid Chromatography (HPLC)
CPT Code:
82491

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Updated: 02/18/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.