Specimen:

Serum

Collection Medium:

Minimum:

Adult preferred minimum: TWO 1 mL aliquots of serum
Adult absolute minimum: 0.5 mL serum
Pediatric Minimum: 0.3 mL serum

Rejection Criteria:

Lipemic, hemolyzed, or contaminated specimens.  Other body fluid 
specimens.

Analytic Time:

1 week

Reference Range:

       Component Name                  Reference Range
Coccidioides Antibody by CF:                < 1:2

Coccidioides Antibody by ID:            None detected

Coccidioides Antibody, IgG by ELISA:
                                       0.9 IV or less = Negative
  Negative-No significant level of Coccidioides IgG antibody detected.
                                       1.0-1.4 IV = Equivocal
  Equivocal-Questionable presence of Coccidioides IgG antibody
  detected.  Repeat testing in 10-14 days may be helpful
                                       1.5 IV or greater = Positive
  Positive-Presence of IgG antibody to Coccidioides detected,
  suggestive of current or past infection.

Coccidioides Antibody, IgM by ELISA:   0.9 IV or less = Negative
  Negative-No significant level of Coccidioides IgM antibody detected.
                                       1.0-1.4 IV = Equivocal
  Equivocal-Questionable presence of Coccidioides IgM antibody
  detected.  Repeat testing in 10-14 days may be helpful.
                                       1.5 IV or greater = Positive
  Positive-Presence of IgM antibody to Coccidioides detected,
  suggestive of current or recent infection.

Test
Limitations:

Note:
For Coccidioides, immunodiffusion (ID) measures IgM antibodies, while 
complement fixation (CF) measures both IgG and IgM. ELISA tests can be 
used to detect both coccidioidal IgG and IgM antibodies. While elevated 
single antibody titers may be diagnostic, paired samples are preferred.  
Acute and convalescent samples (drawn at least 21 days apart), showing 
a fourfold or greater rise in titer, are diagnostic.

Negative fungal serology does not rule out the possibility of current 
infection.

Methodology:

Complement Fixation (CF)
Immunodiffusion (ID)
Enzyme-Linked Immunosorbent Assay (EIA)

CPT Code:

86635 Cocci Ab (CF)
86635 Cocci Ab (ID)
86635 Cocci Ab, IgG (EIA)
86635 Cocci Ab, IgM (EIA)

See also:
Aspergillus Antibody by ID, Serum
Blastomyces Antibody by ID, Serum
Coccidioides Antibodies CSF by CF, ID, ELISA, CSF
Fungal Serology, Serum
Histoplasma Antibodies CF, ID, Serum