The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK

Vitamin D, 25-Hydroxy
Order Code: VITD25
Order Form: A-1a Miscellaneous Request or IPR Req 		  Commercial "Mail-out" Laboratory
6240 RCP
356-3527 
Specimen
 
Plasma
Collection Medium:
Plasma separator tube 


Alternate
Collection Media:


Lavender top tube (EDTA), Light Green top tube (Lithium Heparin), Red 
top tube


Minimum:


Adult Minimum: 1.0 mL plasma
Absolute Pediatric Minimum: 0.3 mL plasma


Analytic Time:


4 working days


Reference Range:


Deficiency:  Less than 20 ng/mL
Insufficiency:  20-29 ng/mL
Optimum Level:  30-80 ng/mL
Possible Toxicity:  Greater than 80 ng/mL


Interpretive Data:


This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and 
vitamin D2, 25-Hydroxy.  Optimum 25-hydroxy vitamin D concentration is 
35 ng/mL or higher.


Methodology:


Chemiluminescent Immunoassay


CPT Code:


82306




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Updated: 08/19/2008

Note: The information contained in this handbook is  
            for use by personnel of University of Iowa Health Care. 
            No other use is implied or intended.