The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


TCRG Clonality by PCR
Order Code: TCELLPCR
Epic Lab Code: LAB2487
Order Form: A-1a Miscellaneous Request or Epic Req
  Molecular Pathology
6004 BT GH
384-9568
Specimen:
Various
Collection Medium:
Lavender top tube (EDTA)
Minimum:
5 ml blood in a lavender top or yellow top, 1 ml bone marrow aspirate 
in a lavender top or yellow top,  3 cu mm of fresh frozen tissue or 
Formalin-fixed, paraffin embedded tissue, body fluids in a lavender top 
or yellow top or lymph node aspirates in RPMI.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Testing Schedule:
Weekly
Analytic Time:
7 working days
Reference Range:
None
Comments:
DNA extracted from blood, bone marrow mononuclear cells or tissue is 
examined for rearrangement of T-cell receptor gamma (TCRG) genes.
Test
Limitations:
Specimens fixed in B5 fixative are unsuitable for testing.
Methodology:
Multiplex Polymerase Chain Reaction (PCR) followed by Fluorescence 
Capillary Electrophoresis
CPT Code:
83890, 83900, 83901, 83909 (x3), 83912
If paraffin block or fresh tissue is submitted, remove CPT code 83890 
and add 83907.

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Updated: 02/15/2007

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.