Specimen:

Whole Blood, Serum and Urine

Collection Medium:

Minimum:

3 full 6 mL pink K2 EDTA top tube
1 full 6 mL red top tube
.5 mL of clean catch urine if testing is needed
  for chlamydia/or gonorrhea

Analytic Time:

1 week

Reference Range:

Antibody to Trypanosoma cruzi (Anti-T.cruzi)
  Negative
Chlamydia trachomatis (Chlamydia)
  Negative
CMV Antibody
  Negative (Positive result suggest current or past infection with
  Cytomegalovirus (CMV).  The presence of Anti-CMV does not assure
  immunity to the disease.)
Antibody to Hepatitis B Core Antigen, Total (detects IgG and IgM)
  Negative
Hepatitis B Surface Antigen
  Non-reactive
Hepatitis C Antibody
  Non-reactive
HIV 1/2 Antibody plus O
  Non-reactive
HTLV I/II Antibody Screen
  Non-reactive
MPX (HIV/HCV/HBV PCR-NAT)
  These 3 tests are done together, they cannot be separated out.
  Non-reactive
  *If the MPX results is negative then the associated comments will
   state that there is no evidence of exposure to HIV-1, M, O, HIV-2,
   HBV, HCV.
  *If the MPX test is positive, the results will indicate a positive.
   Further discriminary testing will subsequently be performed for
   HIV-1, HCV and HBV to identify the positive marker.
Neisseria Gonorrhoeae (Gonorrhea)
  Negative
Syphilis TP (TPPA)
  Non-reactive
West Nile Virus (WNV NAT)
  Negative

Comments:

If you require Antibody to Trypanosoma cruzi (Anti-T.cruzi) testing 
(TCRUZIMN) this must be ordered on the requisition along with the 
ORGTMN.

If you require West Nile Virus nucleic acid testing (WNVNAT) this must 
be ordered on the requisition along with the ORGTMN.

These tests may be ordered only if the patient is part of the donor 
network, sperm, egg or embryo donor, autologous blood donor or stem 
cell donor.

Methodology:

CMV antibody, Antibody to Hepatitis B Core Antigen, Hepatitis B Surface 
Antigen, Hepatitis C Antibody, HIV 1/2 Antibody plus O, CMV-Immucor and 
HTLV I/II Antibody Screen use Enzyme Immunoassay (EIA) methodology for 
testing.

HIV 1/2 plus O series Antibody Immunoblot will be confirmed on any
reactive specimen.  Confirmatory scheme includes the following testing:  
Anti HIV-1 Western Blot (Bio-Rad Western blot), HIV-2 EIA (if Western 
blot negative) (Bio-Rad), and HIV-2 Immunoblot (if HIV-2 EIA positive) 
(Viromed).

MPX series (HIV/HCV/HBV PCR-NAT) and West Nile Virus (NAT) use Nucleic 
Acid Testing (NAT) Roche Molecular method.

Syphilis Treponema Pallidum uses microhemagglutination on the Olympus 
PK system.

Confirmatory Testing:
Hepatitis B Surface Antigen Confirmation:  Ortho Enzyme Immunoassay 
System 3.0

Hepatitis C Antibody Confirmation:  Recombinant Immunoblot Assay (HCV
RIBA:  Chiron-SIA-v.3.0)

HIV-1 Group O or HIV-2.  These results should be evaluated in the 
context of the individual's risk factors and other clinical findings.
  MPX: (Roche Multiplex PCR)
  HBV-PCR: (Roche HBV-PCR)
Discriminatory testing performed at Lifesource Testing Laboratory
  HCV-PCR: (Roche HCV-PCR)
Discriminatory testing performed at Lifesource Testing Laboratory
  HIV-1PCR: (Roche HIV-1-PCR)
Discriminatory testing performed at Lifesource Testing Laboratory

Syphilis Treponema Pallidum (Syphilis TP) confirmatory testing:  
Fluorescent Treponemal Antibody (FTA-ABS) sent to Viromed Laboratory.

Chlamydia trachomatis (Chlamydia):  uses the Gen-Probe Amptima Combo 2 
kit; transferred by Mailouts to this kit for submission to reference 
laboratory

Neisseria Gonorrhoeae (Gonorrhea):  uses the Gen-Probe Amptima Combo 2 
kit; transferred by Mailouts to this kit for submission to reference 
laboratory.

CPT Code:

Antibody to Trypanosoma cruzi (Anti-T.cruzi) EIA screen: CPT = 86753
  Confirmation testing RIPA confirmed CPT = 86753

Chlamydia trachomatis CPT = 87491

CMV Antibody CPT = 86644

Hepatitis B Core Antigen  CPT = 86704

Hepatitis B Surface Antigen  CPT = 87340
  (Hepatitis B Surface Antigen Confirmatory testing done
   automatically if positive.  CPT code =  87340)

Hepatitis C Antibody CPT = 86803
  (Hepatitis C Antibody Confirmatory testing done automatically
   if positive.  CPT code = 86804)

HIV 1/2 Antibody plus O CPT = 86703
  (HIV 1/2 Antibody Immunoblot will be confirmed on any reactive
   specimen; CPT code = 86689.  Confirmatory scheme includes the
   following testing:  Anti HIV-1 Western Blot (Bio-Rad),
   HIV-2 EIA (if Western blot negative--Bio-Rad), and
   HIV-2 Immunoblot (if HIV-2 EIA positive--Viromed).

MPX (HIV/HCV/HBV PCR-NAT) - Combination Test as a screen has the 
following CPT's:
   HIV = 87535
   HCV = 87521
   HBV = 87516
Confirmatory testing will be performed on all positive samples using 
the following CPT's:
   HIV = 87535
   HCV = 87521
   HBV = 87516
Additional charges apply to confirmatory testing.

HTLV I/II Antibody:  HTLV I CPT code = 86687; HTLV II CPT code =
  86688.  HTLV I/II virus type I/II antibody confirmation will be
  performed to confirm presence of antibodies to HTLV-I HTLV-II in
  samples repeatedly reactive by EIA screening test.  Second
  manufacturer's EIA method CPT codes = 86687 and 86688.

Neisseria Gonorrhoeae CPT = 87491

Syphilis TP CPT code = 86592  If positive, confirmatory
  test is performed FTA-ABS = 86781 at Viromed.

West Nile Virus (WNV NAT) CPT code = 87798