Specimen:

Serum

Collection Medium:

Minimum:

Adult minimum:  1.0 mL serum
Absolute Adult minimum:  0.2 mL serum

Pediatric minimum: 0.1 mL serum

Rejection Criteria:

Lipemic, hemolyzed, icteric, turbid, bacterially contaminated, or 
heat-inactivated specimens.  Plasma specimens.

Analytic Time:

1 week

Reference Range:

Adenovirus Antibody, IgG
  Negative:  0.89 IV or less
      No significant level of adenovirus IgG antibody detected.

  Equivocal:  0.90 - 1.10 IV
      Questionable presence of adenovirus IgG antibody detected.
      Repeat testing in 10-14 days may be helpful.

  Positive:  1.11 IV or greater
      IgG antibodies to adenovirus detected, which may suggest current
      or past infection.

Adenovirus Antibody, IgM
  Negative:  0.89 IV or less
      No significant level of adenovirus IgM antibody detected.

  Equivocal:  0.90 - 1.10 IV
      Questionable presence of adenovirus IgM antibody detected.
      Repeat testing in 10-14 days may be helpful.

  Positive:  1.11 IV or greater
      IgM antibodies to adenovirus detected, which may suggest current
      or recent infection.

Test
Limitations:

Adenoviruses are DNA viruses responsible for 4-5 percent of all 
respiratory illnesses. There are 41 known serotypes with diverse 
clinical manifestations, ranging from acute pharyngoconjunctival fever 
and acute respiratory outbreaks to epidemic keratoconjunctivitis and 
intestinal tract infections. Because of the varied serotypes, 
adenoviruses cause infections year-round. Although adenovirus 
infections are rarely fatal, about 50 percent of infants and young 
children have prolonged intermittent disease and fatal adenovirus 
infections can occur in infants and immunocompromised adults.

Most cases are diagnosed on clinical grounds alone. Viral culture can 
be used in selected clinical settings. Serologic testing usually relies 
upon the demonstration of antibodies to group-specific antigens. 
Individual serotype specificity is seldom required.

Methodology:

Enzyme-Linked Immunosorbent Assay

CPT Code:

86603(x2)

See also:
Adenovirus Antigen Detection by DFA, Nasopharyngeal Swab/Aspirate/Wash, Tracheal Aspirate, Bronchoalveolar Lavage, Bronchial Wash
Adenovirus Culture, Various