The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Vitamin B1 (Thiamine)
Order Code: VTB1B
Order Form: A-1a Miscellaneous Request or IPR Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen
Whole Blood
Collection Medium:
Green top tube (Na Heparin)
Alternate
Collection Media:
Lavender top tube (EDTA), Light Green top tube (Lithium Heparin), Pink 
top tube (EDTA sprayed)
Minimum:
Adult preferred minimum: 3 mL whole blood from green top (NA Heparin) 
tube.
Adult absolute minimum: 1 mL whole blood from green top (NA Heparin) 
tube.
Pediatric minimum: 0.7-1.0 mL whole blood from lavender top (EDTA) tube
Delivery Instructions:
Wrap specimen in aluminum foil to protect from light.  
Deliver to laboratory immediately after collection.
Specimen
Instructions:
Whole Blood is sample of choice.
Analytic Time:
6 days
Reference Range:
70-180 nmol/L
Interpretive Data:
The concentration of thiamine diphosphate (TDP), the primary active 
form of vitamine B1, is measured in this assay.  Approximately 90% of 
vitamine B1 present in whole blood is TDP.  Thiamine and thiamine 
monophosphate, which comprise the remaining 10%, are not measured.
Methodology:
High Performance Liquid Chromatography
CPT Code:
84425
 
See Additional Information:
Specimens Requiring Immediate Delivery

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Updated: 08/19/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.