The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Viral Culture

Order Form: A-1a Clinical Microbiology Laboratory or IPR Req
  Microbiology
BT 6004
356-2591 (0700-2300)Bacteriology/Virology
Section

356-3527 (2300-0700)
Core Lab
Rejection Criteria:
Sputum specimens.
Delivery Instructions:
Mix well and place on wet ice.  Keep on ice and deliver to
laboratory immediately.
Testing Schedule:
0700-1630, 7 days a week, including holidays.
Comments:
See specific viral agents for more details.
A.  Biopsy or tissue - immerse in transport media.  Available from
Hospital Stores (923740).
B.  Blood or buffy coat culture - collect 10 ml of blood in a
lavender (EDTA) tube.
C.  Bone Marrow - collect in a lavender (EDTA) tube.
D.  CSF - collect 1-5 ml in a sterile container.
E.  Nasopharyngeal wash - inoculate 3 ml of normal sterile saline
into the nasal passage with a bulb syringe while the patients head is
tilted back.  Aspirate 1-2 ml into a sterile container.
F.  Respiratory secretions - collect specimens in a sterile container.
Sterile container for bronchial washings and bronchoalveolar lavage
specimens.  Mucous trap or delee trap for tracheal aspirates and nasal
washings.
G.  Swab - Transport media available from Hospital Stores (923740).
H.  Throat wash - Have patient gargle with 3-5 ml of normal sterile
saline.  Collect wash in a sterile specimen container.
I.  Vesicle - Expose the base of the lesion by lifting the cap of the 
vesicle or ulcer.  Use swab from viral/chlamydia transport kit 
(923740), swab entire base of the lesion firmly to collect cellular 
material (without causing bleeding).  Place swab in viral transport 
media.
J.  Urine - collect 1-10 ml of clean voided midstream morning urine
collected in a sterile container.
K.  Sterile body fluids and tissues.
 
See Additional Information:
Specimens Requiring Immediate Delivery

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Updated: 07/08/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.