The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Varicella-Zoster PCR
Order Code: VZPCR
Order Form: A-1a Miscellaneous Request or IPR Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen
CSF, Ocular specimen, Tissue Biopsy, Vesicle fluid
Collection Medium:
CSF container
Minimum:
1 mL CSF, ocular specimen in sterile container or tissue biopsy

Pediatric Minimum: 0.25 mL
Rejection Criteria:
Non sterile or leaking containers, heparinized or hemolyzed specimens, 
tissue in formalin or other preservatives.
Analytic Time:
4 days
Reference Range:
Reference Range:  Negative = VZV DNA not detected by PCR
                  Positive = VZV DNA detected by PCR
A negative result does not rule out the presence of PCR reaction 
inhibitors in the patient specimen or VZV DNA in concentrations below 
the level of detection by the assay.
Comments:
Specimen source must be recorded on requisition.

Freeze tissue immediately.  Transport fresh snap frozen tissue on dry 
ice.

Transport vesical fluid or tissue biopsy in viral transport media.
Methodology:
Polymerase Chain Reaction
CPT Code:
87798

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Updated: 08/12/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.