Specimen

Serum

Collection Medium:

Alternate
Collection Media:

Light Green top tube (Lithium Heparin)

Minimum:

Adult preferred minimum: 1 mL serum or lithium heparin plasma collected 
from 3 mL whole blood

Adult absolute minimum: 0.6 mL serum or lithium heparin plasma 
collected from 1.8 mL whole blood

Pediatric minimum: 0.4 mL serum or lithium heparin plasma collected 
from 1.2 mL whole blood

Rejection Criteria:

EDTA plasma

Specimen
Instructions:

Specimen should be collected between 6:00 - 10:00 a.m.

Not recommended for females or children.  For females or children, see 
FTSTFC.

Analytic Time:

2 working days (upon receipt)

Reference Range:

Testosterone, Percentage Free
18 years and older:  1.6-2.9%

Sex Hormone Binding Globulin
Age                       Male             Female
1-30 days             13-85 nmol/L     14-60 nmol/L
31-364 days           70-250 nmol/L    60-215 nmol/L
1-3 years:            50-180 nmol/L    60-190 nmol/L
4-6 years:            45-175 nmol/L    55-170 nmol/L
7-9 years:            28-190 nmol/L    33-170 nmol/L
10-12 years:          23-160 nmol/L    17-155 nmol/L
13-15 years:          13-140 nmol/L    11-120 nmol/L
16-17 years:          10-60 nmol/L     19-145 nmol/L
18 years and older:   11-80 nmol/L     30-135 nmol/L
Tanner Stage I:       26-286 nmol/L    30-173 nmol/L
Tanner Stage II:      22-169 nmol/L    16-127 nmol/L
Tanner Stage III:     13-104 nmol/L    12-98 nmol/L
Tanner Stage IV:      11-60  nmol/L    14-151 nmol/L
Tanner Stage V:       11-71  nmol/L    23-165 nmol/L

Testosterone Free, Adult Male
14-15 years:         3-138 pg/mL
16-17 years:        38-173 pg/mL
18 years and older: 47-244 pg/mL
Tanner Stage IV:    35-169 pg/mL
Tanner Stage V:     41-239 pg/mL

Testosterone, Adult Male
14-15 years:         33-585 ng/dL
16-17 years:        185-886 ng/dL
18-39 years:        400-1080 ng/dL
40-59 years:        350-890 ng/dL
60 years and older: 350-720 ng/dL
Tanner Stage IV:    165-854 ng/dL
Tanner Stage V:     194-783 ng/dL

Interpretive Data:

The concentration of free testosterone is derived from a mathematical 
expression based on the constant for the binding of testosterone to sex 
hormone binding globulin.

Methodology:

Electrochemiluminescent Immunoassay

CPT Code:

84403 Testosterone, 84270 Sex Hormone Binding Globulin

See also:
Sex Hormone Binding Globulin, Serum or Plasma
Testosterone, Free and Total, Females or Children, Serum
Testosterone, Total, Pediatric, Serum
Testosterone, Total, Plasma