The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Mycobacterial Culture
Order Code: C AFB
Epic Lab Code: LAB2208
Order Form: A-1a Clinical Microbiology Laboratory or Epic Req
  Microbiology
BT 6004
356-2591 (0700-2300)
Bacteriology/Virology Section

356-3527 (2300-0700)
Core Lab
Collection Medium:
Sterile container
Delivery Instructions:
Deliver to laboratory within 30 minutes of collection. If transport is 
delayed, specimens nust be refrigerated and delivered within 1 day of 
collection.
Testing Schedule:
0700-1630, 7 days a week, including holidays.
Analytic Time:
Cultures are held for 6 weeks.
Comments:
A.  Blood - media and instructions available upon request from the
Microbiology Lab.  A transfer device will be distributed with these 
culture bottles.  Test available for limited patient populations only.

B.  Sputum - collect an early morning specimen on three consecutive
days for the diagnosis of pulmonary tuberculosis.  Collect 5-15 mL in a 
sterile container.  Pooled specimens are unacceptable.

C.  Swabs are suboptimal for the recovery of mycobacteria since they 
provide limited material and the hydrophobicity of the mycobacterial 
cell envelope often compromises a transfer from swabs onto media. See 
bacterial culture for collection and transport of all other specimen 
types.
Methodology:
Standard culture media and/or semi-automated radiometric detection
CPT Code:
87116
 
See also:
Bacterial Culture

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Updated: 10/06/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.