Specimen

Various

Collection Medium:

Alternate
Collection Media:

Green top tube (Na Heparin), Lavender top tube (EDTA)

Minimum:

Adult
Peripheral Blood: 10 ml; Bone Marrow:  2-4 mL yellow top tube (ACD 
solution A)

Pediatric
Peripheral Blood:  2-5 mL; Bone Marrow:  2-5 mL lavender top tube 
(EDTA) or green top tube (Na Heparin).

Fluids and Tissue: Dispense sample into vial of RPMI-1640 tissue 
culture media.

Delivery Instructions:

Keep at room temperature.  Do not refrigerate.

Testing Schedule:

0800-1630 Monday through Friday.  For additional services, 
contact Clinical Pathology Resident on-call at pager #3404.

Analytic Time:

2 days

Reference Range:

Antibodies routinely included are: CD2, CD3, CD5, CD10, CD11c, CD14, 
CD19, CD20, CD22, CD23, CD25, CD38, CD45, CD103, CD138, FMC7, Heavy 
chains, Kappa and Lambda. Antibodies will also identify cases of 
Prolymphocytic and Hairy Cell Leukemia.

If clinical or morphologic features suggest a "T" or "NK" lymphocyte 
disorder, then the following additional antibody combinations are 
performed: CD3/CD4/CD8, CD7/CD5/HLA-DR, CD14/CD45, CD25/CD2/CD3, 
CD16/CD56/CD19, CD57/CD8/CD3, TCR alpha-beta/delta-gamma/CD3.

The pathologist will provide an interpretative report.

Comments:

Please state the clinical question to be answered on the requisition. 
Specimens accepted from Monday 0800 until Friday 1630.  Clinical 
Pathology resident should be contacted if studies are needed emergently 
at other times.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

88184, 88185(x20) technical; 88189 professional; T cell or NK cell 
disorder: 88184, 88185(x15) technical; 88188 professional

See Additional Information:
Specimens Requiring Immediate Delivery